Molecular epidemiology of Pseudomonas aeruginosa isolated from lower respiratory tract of ICU patients

Abstract Lower respiratory tract infections (LRTIs) caused by Pseudomonas aeruginosa are the most common infection among hospitalized patients, associated with increased levels of morbidity, mortality and attributable health care costs. Increased resistant worldwide has been quite meaningful to especially in intensive unit (ICUs). Different species exhibit different genetic profile varied drug resistance. The present study determines molecular epidemiology through DNA fingerprinting method resistance P. isolated from patients LTRIs admitted ICU. A total 79 LRTIs ICU were characterized Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic (RAPD) Repetitive Extrapalindromic PCR (REP-PCR). Antibiotic was determined minimum inhibitory concentration (MIC) assay while MDR genes, viz, blaTEM, blaOXA, blaVIM, blaCTX-M-15 detected polymerase chain reaction (PCR). Of 137 sp 57.7% isolates reported be aeruginosa. overall prevalence all included 34.5%. RAPD analysis yielded 45 patterns 72 clusters 57% 100% similarity level. RFLP 8 14 76% REP 37 65 56% There no correlation observed between three methods. Predominant (46.8%) amikacin. isolates, 60.8% positive for blaTEM gene 39.2% blaOXA gene. predominantly difference level methods indicates that there is high inter-strain variability. variability we should continuously monitor trend antibiotic